SAMEER KALGHATGI

           

Current News and Updates

CV and Resume updated to the latest versions

May 6th, 2012

Resume and CV updated on May 6th 2012. Check out the latest versions on the Resume and CV page respectively. To download the Resume and CV in PDF or Word format please go to the Downloads page.

 

Journal article published online by Plasma Processes and Polymers

April 16th, 2012

My paper titled "DNA Damage in Mammalian Cells by Non-thermal Atmospheric Pressure Microsecond Pulsed Dielectric Barrier Discharge Plasma is not Mediated by Ozone" was published online on April 16th 2012 in Plasma Processes and Polymers. Click here to view the early view article online. Download PDF

Abstract

Non-thermal dielectric barrier discharge (DBD) plasma is now being widely developed for various clinical applications, but the mechanisms of interaction of such plasmas with mammalian cells are still not well understood. It is known that DBD plasma produces ozone and it is possible that the effects of plasma may be mediated purely by ozone. Although typically requiring much higher treatment time than the typical plasma treatment times reported here, ozonation is, in fact, widely employed to kill microorganisms in water and is also used for wound healing. It has been shown earlier that plasma treatment of mammalian cells submerged in a shallow layer of culture medium can result in dose dependent DNA damage. We wanted to understand the role of ozone in mediating the interaction of non-thermal plasma DBD plasma with mammalian cells. Upon comparing plasma treatment of mammalian breast epithelial cells with ozone treatment we found that ozone treatment is qualitatively different from non-thermal DBD plasma and in fact does not play a role in mediating the observed effects of plasma on mammalian cells.

 

Journal article published online by the Journal of Surgical Research

March 10th, 2012

A paper entitled "Porcine intact and wounded skin responses to atmospheric non-thermal plasma" where I am the second author was epublished online before print by the Journal of Surgical Research.

Abstract

Thermal plasma is a valued tool in surgery for its coagulative and ablative properties. We suggested through in vitro studies that nonthermal plasma can sterilize tissues, inactive pathogens, promote coagulation, and potentiate wound healing. The present research was undertaken to study acute toxicity in porcine skin tissues. We demonstrate that floating electrode-discharge barrier discharge (FE-DBD) nonthermal plasma is electrically safe to apply to living organisms for short periods. We investigated the effects of FE-DBD plasma on Yorkshire pigs on intact and wounded skin immediately after treatment or 24h posttreatment. Macroscopic or microscopic histological changes were identified using histological and immunohistochemical techniques. The changes were classified into four groups for intact skin: normal features, minimal changes or congestive changes, epidermal layer damage, and full burn and into three groups for wounded skin: normal, clot or scab, and full burn-like features. Immunohistochemical staining for laminin layer integrity showed compromise over time. A marker for double-stranded DNA breaks, γ-H2AX, increased over plasma-exposure time. These findings identified a threshold for plasma exposure of up to 900s at low power and <120s at high power. Nonthermal FE-DBD plasma can be considered safe for future studies of external use under these threshold conditions for evaluation of sterilization, coagulation, and wound healing.

 

December 2nd, 2011

Author proofs received for my paper titled "DNA Damage in Mammalian Cells by Non-thermal Atmospheric Pressure Microsecond Pulsed Dielectric Barrier Discharge Plasma is not Mediated via Lipid Peroxidation".

 

Journal article accepted for publication in the Plasma Medicine Journal

September 12th 2011

My paper titled "DNA Damage in Mammalian Cells by Non-thermal Atmospheric Pressure Microsecond Pulsed Dielectric Barrier Discharge Plasma is not Mediated via Lipid Peroxidation" was accepted for publication on Sep 9th 2011 in the Plasma Medicine Journal.

Abstract:

Lipid peroxidation in mammalian cells by non-thermal atmospheric pressure microsecond pulsed dielectric barrier discharge plasma was investigated. It has been shown earlier that plasma treatment of mammalian cells submerged in a shallow layer of culture medium can result in dose dependent DNA damage. We show that non-thermal plasma induces lipid peroxidation, measured by release of Malondialdehyde (MDA), in cells treated under medium and not under phosphate buffered saline (PBS). N-Diphenyl-Phenyl-enediamine (DPPD), a lipophilic antioxidant was used to block non-thermal plasma induced lipid peroxidation. We show that non-thermal plasma induces lipid peroxidation in mammalian cells, but non-thermal plasma induced lipid peroxidation does not lead to DNA damage in mammalian cells. Thus, one needs to consider other possible mechanisms for plasma induced DNA damage in mammalian cells such as ROS signaling or active transport of long-lived organic hydroperoxides

February 3rd, 2011

Biodata, Resume and CV updated on Feb 3rd 2011. Check out the latest versions on the Biodata, Resume and CV page respectively. To download the Resume and CV in PDF or Word format please go to the Downloads page.

 

Presented a talk at the BMES Annual Meeting in Hartford, CT

Oct 15th 2011

Presented a talk entitled "Bactericidal Antibiotics induce Oxidative stress via Mitochondrial Dysfunction in Mammalian Cells'" at the BMES Annual meeting in Hartford, CT

Journal article published online in PLoS One

January 21st, 2011

My paper titled "Effects of Non-Thermal Plasma on Mammalian Cells" was published online on January 21st, 2011 in PLos One. Click here to view the article. To download the PDF click here.

Abstract

Thermal plasmas and lasers have been widely used in medicine to cut, ablate and cauterize tissues through heating; in contrast, non-thermal plasma produces no heat, so its effects can be selective. In order to exploit the potential for clinical applications, including wound healing, sterilization, blood coagulation, and cancer treatment, a mechanistic understanding of the interaction of non-thermal plasma with living tissues is required. Using mammalian cells in culture, it is shown here that non-thermal plasma created by dielectric barrier discharge (DBD) has dose-dependent effects that range from increasing cell proliferation to inducing apoptosis. It is also shown that these effects are primarily due to formation of intracellular reactive oxygen species (ROS). We have utilized c-H2AX to detect DNA damage induced by non-thermal plasma and found that it is initiated by production of active neutral species that most likely induce formation of organic peroxides in cell medium. Phosphorylation of H2AX following non-thermal plasma treatment is ATR dependent and ATM independent, suggesting that plasma treatment may lead to replication arrest or formation of single-stranded DNA breaks; however, plasma does not lead to formation of bulky adducts/thymine dimers.

Journal article published online in the Annals of Biomedical Engineering

November 3rd, 2010

My paper titled "Non-Thermal Plasma Induces Apoptosis in Melanoma Cells via Production of Intracellular Reactive Oxygen Species" was published online on November 3rd, 2010 in the Annals of Biomedical Engineering. Click on the paper to download the article.

 

Outstanding Research Award

May 21st 2010

I was extremely pleased to know that I was awarded the Highly Commended Citation for the 2010 Drexel University Outstanding Research Award by the Office of Graduate Studies. It is indeed a very proud moment. The awards ceremony will be held on Jun 1st 2010. Check out the details here. Research Awards Competition

 

D-Day: Ph.D. Dissertation Defense

March 15th 2010

After five and a half years, this day is finally here. I defend my PhD on Monday, Mar 15th 2010. Here are the complete details of my defense. One long, memorable and eventful journey comes to an end.

ECE Ph.D. Dissertation Defense


When: Monday, March 15, 2010 10:00 AM-11:30 AM
Where: Bossone 709, Bossone Research Enterprise Center, Drexel University Main Campus, 3120-24 Market St, Philadelphia, PA-19104

Abstract:
Thermal plasmas and lasers have been widely used in medicine to cut, ablate and cauterize tissues through heating; in contrast, non-thermal plasma produces various highly active molecules and atoms without heat. As a result, its effects on living cells and tissues could be selective and tunable. This makes non-thermal plasma very attractive for medical applications. However, despite several interesting demonstrations of non-thermal plasma in blood coagulation and tissue sterilization, the biological and physical mechanisms of its interaction with living cells are still poorly understood impeding further development of non-thermal plasma as a clinical tool. Although several possible mechanisms of interaction have been suggested, no systematic experimental work has been performed to verify these hypotheses. Using cells in culture, it is shown in this work that non-thermal plasma created by dielectric barrier discharge (DBD) has dose-dependent effects ranging from increasing cell proliferation to inducing apoptosis which are consistent with the effects of oxidative stress. DNA damage is chosen as a marker to assess the effects of oxidative stress in a quantitative manner. It is demonstrated here that plasma induced DNA damage as well as other effects ranging from cell proliferation to apoptosis are indeed due to production of intracellular reactive oxygen species (ROS). We found that DNA damage is initiated primarily by plasma generated active neutral species which cannot be attributed to ozone alone. Moreover, it is found that extracellular media and its components play a critical role in the transfer of the non-thermal plasma initiated oxidative stress into cells. Specifically, it is found that the peroxidation efficiency of amino acids is the sole predictor of the ability of the medium to transfer the oxidative stress induced by non-thermal plasma. Phosphorylation of H2AX, a DNA damage marker, following plasma treatment is found to be ATR dependent and ATM independent, suggesting that non-thermal plasma may induce formation of bulky lesions unlike ionizing radiation (IR) or H2O2 which primarily produce DNA double strand breaks. Moreover, it is found that the pathway by which plasma generated oxidative stress is transferred across cellular membranes does not involve lipid peroxidation by-products, although lipid peroxidation does occur.

 

Non-Thermal Plasma for Hospital Clean Hands, Without All the Scrubbing

February 14th 2010

HOSPITAL workers often have to wash their hands dozens of times a day — and may need a minute or more to do the process right, by scrubbing with soap and water. But new non-thermal plasma devices could reduce the task to just four seconds, cleaning even hard-to-reach areas under fingernails....read more

Images copyright of

Featured in Fall/Winter Edition of ASME Bioengineering Division's Newsletter

January 14th 2010

My poster presented at the Summer Bioengineering Conference in Jun 2009 at Lake Tahoe was featured in the ASME BED's Fall/Winter newsletter. Check out the newsletter @ http://files.asme.org/Divisions/BED/21517.pdf and I was featured on pages 10 and 11 and my poster was featured on page 17.

Journal article published online in the Annals of Biomedical Engineering

December 21st 2009

My paper titled "Endothelial Cell Proliferation is Enhanced by Low Dose Non-Thermal Plasma through Fibroblast Growth Factor-2 Release" was published online on Dec 15th 2009 in the Annals of Biomedical Engineering. Click on the paper to download the article.

Paper accepted in the Annals of Biomedical Engineering

December 3rd 2009

My paper titled "Endothelial Cell Proliferation is Enhanced by Low Dose Non-Thermal Plasma through Fibroblast Growth Factor-2 Release" was today accepted for publication in the Annals of Biomedical Engineering. Preprints are awaited. To read the paper please follow the links below.

Accepted Draft - PDF

Pre-prints - PDF

Paper submitted to the Proceedings of the National Academy of Science

December 2nd 2009

My paper entitled "Effects of Non-Thermal Plasma on Mammalian Cells" was submitted today for publication in the Proceedings of the National Academy of Science. Keep a look out for the accepted draft at this site.

Accepted Draft - Under Review

Pre-Prints - Awaiting acceptance

Toxicity of Non-Thermal Plasma Treatment of Tissue

November 23rd 2009

Added a section on animal studies to test the toxicity of non-thermal plasma treatment of intact tissue and wounded skin to the Research page of my website. Please visit the Tissue Toxicity page for more details.

Recent Updates

Resume, CV and biodata modified on Nov 23rd 2009. Please find my modified CV, Resume and Biodata by clicking on the links. To download my CV and resume in word or PDF formats please visit the Downloads page.

Paper submitted to the Proceedings of the National Academy of Science

October 30th 2009

My paper entitled "DNA Damage in Mammalian Cells by Non-Thermal Atmospheric Pressure Microsecond Pulsed Dielectric Barrier Discharge Plasma is not mediated via Lipid Peroxidation" was submitted for publication in the Special Issue of the IEEE Transactions on Plasma Science focusing on Non-Thermal Plasma and its applications in biology and medicine. Keep a lookout for the accepted manuscript

Accepted Draft: Under Review

Pre-print: awaiting acceptance

Recent Updates

Resume, CV and biodata modified on Aug 23rd 2009. Please find my modified CV, Resume and Biodata by clicking on the links. To download my CV and resume in word or PDF formats please visit the Downloads page.

Website Re-launch

Finally launched the new and improved website after working on it for more than a month. The old website was fine, but I thought it was time for something fresh and of course I wanted to move to xHTML with CSS from plain vanilla HTML. The new website also offers more information and details on my research and features a Google search bar to search within my website and a new flash photo gallery. Check it out here www.sameerkalghatgi.com/index.html.

 

17th IEEE Pulsed Power Conference, Jun 29th - Jul 2nd 2009, Washington DC, USA

Pictures from the recently concluded PPC 2009. Left: Presenting my poster on toxicity of non-thermal plasma treatment of living tissue at the recently concluded 17th IEEE International Pulsed Power Conference, Jun 29th - Jul 2nd 2009, Washington DC. Right: Attending a session at the historic Mayflower Hotel in Washington DC, the venue of the 2009 PPC.

ASME Summer Bioengineering Conference, Jun 17th - 21st 2009, Lake Tahoe, CA, USA

Pictures from the recently concluded Summer Bioengineering. Clockwise from top: Kivilcim, Danni, Steve and me with our certificates, Presenting my poster on plasma enhanced proliferation of endothelial cells via release of FGF-2. The poster won a honorable mention in the tissue engineering section at the PhD level student paper competition

36th International Conference on Plasma Science, May 31st - Jun 4th 2009, San Diego, CA, USA

Pictures from the recently concluded ICOPS in San Diego. Clockwise from top: Presenting my invited talk on Plasma Enhanced Endothelial Cell Proliferation, Explaining my poster on plasma induced apoptosis in melanoma cells to a conference attendee, Presenting my poster on plasma induced apoptosis in melanoma cells and relaxing outside the conference hotel after completing all the talks and posters

Drexel University Honors Day Award Ceremony

Receiving a scroll for the "2008-09 Drexel University George Hill Jr. Endowed Fellowship" from Dr. Selcuk Guceri, the Dean of the College of Engineering, Drexel University on 17th February 2009.

Published a chapter in a book

Plasma Assisted Decontamination of Biological and Chemical Agents (NATO Science for Peace and Security Series A: Chemistry and Biology) (Paperback)

by Selçuk Güçeri (Editor), Alexander Fridman (Editor), Katie Gibson (Adapter), Christine Haas (Adapter)

Applications of Non Thermal Atmospheric Pressure Plasma in Medicine, S. Kalghatgi, D. Dobrynin, G. Fridman, M. Cooper, G. Nagaraj, L. Peddinghaus, M. Balasubramanian, K. Barbee, A. Brooks, V. Vasilets, A. Gutsol, A. Fridman, and G. Friedman, NATO Advanced Study Institute on Plasma Assisted Decontamination of Biological and Chemical Agents (Springer, Cesme-Izmir, Turkey, 2007), p. 173 - 181.

Jorge Cham @ Drexel

The Triangle, Drexel University's weekly newspaper, published an article on Jorge Cham @ Drexel in their Feb 27th weekly edition. Here is a brief transcript from that article.

"Comic author Jorge Cham had a book signing and discussion about graduate life in academia Feb. 25. Cham is the author of "Piled Higher and Deeper," a comic strip about life in a university. "All the comics were about undergraduates. There needed to be one about the pressures and hardships of graduate life," Cham said. Cham said it's a way of bringing graduate students together. He said graduate life can be isolating and students need other people to relate with...." read more

©2009 The Triangle

 

Jorge Cham @ Drexel

Jorge Cham, the author of PhD comics was hosted by the Graduate Student Association at Drexel University on 25th February 2009. He gave his very entertaining talk on "The Power of Procrastination" followed by book signing of his three books. Check out some of the snaps taken at the event. Click on the image to see more snaps.

The GSA Board with Jorge. From L-R: Chris (VP), Sara (Prez), Dr. Jorge Cham, Jackie (Secretary) and me (Treasurer)

 

PHD comics@ Drexel!

GSA Presents Jorge Cham, Creator of PhD Comics

Lecture and Book signing! Invite your friends!
Host:          
Graduate Student Association @ Drexel University
Date:
Wednesday, February 25, 2009
Time:
12:00pm - 3:00pm
Location:
Bossone Lobby and Mitchell Auditorium
City/Town:
Philadelphia, PA

Description

Join us for a fun filled afternoon of grad student humor as the creator of "Piled Higher and Deeper" delivers his popular talk, "The Power of Procrastination".

11.30am - Start with book signing.
12:00pm - Lunch.
12.30pm - Talk.
01.30pm - Additional book signing time

 

Updated CV available online on the Resume page

I have recently updated my CV to reflect the latest publications and conference oral talks and poster presentations Please download my updated CV in either PDF or word format here

 

Abstract Accepted at the 19th International Symposium on Plasma Chemistry

An abstract submitted to the 19th International Symposium on Plasma Chemistry (ISPC), Jul 26th-Jul 31st, Bochum, Germany has been selected for a poster presentation.

Title: Low Dose Non-Thermal Plasma Interacts with Mammalian Cells Indirectly through Modification of the Cell Culture Medium

S. Kalghatgi, C. Kelly, E. Cerchar, A. Fridman, J. Azizkhan-Clifford and G. Friedman

 

1474 Megapixel (1.5 Gigapixels) Photo Taken During Obama's Inauguration

If you have been following Obama's election and the subsequent inauguration on 20th January, here is a phenomenal 1474 Megapixel panoramic photograph taken by David Bergman from the media platform. Click on the image to see the actual photo which allows you to zoom and pan to see the most amazing details. This remarkable photo is a panoramic composite of 220 individual photos–quite something. Check it out

To know more about how he managed to take this photo click here.

 

Recipient of the 2009 Drexel University George Hill Jr. Endowed Fellowship

click on the images to see a larger picture

 

Plasma Technology is licensed

Drexel University’s plasma medicine technologies developed in the A.J. Drexel Plasma Institute, were licensed to Plasma Technologies, Inc.(PTI), a Texas-based company that intends to design, patent and market medical devices related to wound healing and care, prevention of hospital borne infections, and other medical applications. The technology may have near term impact not only in civilian healthcare settings, but also on the U.S. military applications........read more. Additional information is available here and here

 

Best Student Paper Award at the 35th IEEE International Conference on Plasma Science (ICOPS 2008)

click on the images to see a larger picture.

1. With the conference chair Dr. Thumm after receiving the award. 2. With my co-advisors Dr. Gutsol and Dr. Vasilets and fellow graduate student Moogega

 

A. J. Drexel Plasma Institute established

A few years ago, a researcher at Drexel University accidentally cut his finger and exposed it to plasma, a fourth state of matter created by ionizing gas.To everyone’s surprise, the blood from the cut coagulated. “We said, ‘OK, this is very interesting, maybe we can help somebody with this,’” said Gary Friedman, a professor of electrical and computer engineering and a professor of surgery at Drexel....read more

 

A new book on Plasma Chemistry by Dr. Alexander Fridman

Dr. Alexander Fridman, MEM professor and director of the A.J. Drexel Plasma Institute, has released the first comprehensive book ever published on plasma chemistry. The book titled Plasma Chemistry, provides a fundamental introduction to all aspects of modern plasma science...read more

 

Commentary on Plasma in France by Dr. Alexander Fridman

A short commentary (in French) on non-thermal plasma by Dr. Aleander Fridman on French National TV...check it out here

 

A New Kind of Plasma in Medicine

— Imagine performing surgeries without scalpels or without cutting the skin. Imagine treating diabetic wounds without antibiotics, but rather with electrical charges. This is what plasma technology can do. It's not the kind of plasma found in the blood but the kind found in plasma televisions. Plasma is gas......read more

 

Plasma Medicine a major research initiative at Drexel University

The goal of this Major Research Initiative is to build on our recent achievements in the application of plasma science to biology and medicine. These breakthrough developments grew out of productive collaborations between engineers, biological scientists and physicians.  The existence of this energetic and mutually beneficial collaborative group provides a unique opportunity for revolutionary research in the application of plasma to selectively kill micro-organisms while sparing living mammalian tissues, and possibilities in therapeutic manipulation of proteins as in the process of coagulation or triggering of apoptosis pathways. These highly promising avenues of research...read more

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Resume and Full CV

Updated on Jun 2, 2009

To view or download my resume, CV or Biodata  please visit these links

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Publications

Updated on Jun 2, 2009

Find out more about my  talks, papers and other research publications

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Areas of Research   

Updated On Jun 2, 2009

Please read about my   current areas of research    by visiting the links below

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Current Happenings

Updated On Jun 2, 2009

View photos from my latest travel or read more about current happenings in my life

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