Resume

Sameer Kalghatgi, Ph. D.

1.  Exceptional skills in in vitro primary tissue and mammalian cell culture, DNA damage detection and repair, promotion and inhibition of angiogenesis, apoptosis, cellular signaling and phosphorylation, RNAi, Microarrays, genomic DNA sequencing, RNA and DNA Extraction, Western blotting, RT²-PCR, immunohistochemistry, histology and FACS along with design, implementation & execution of in vivo animal studies.

2.  Energetic results oriented leader, innovative problem solver, and an enthusiastic team player capable of working independently or in a challenging fast-paced team-centered multidisciplinary environment. Critical thinker with a demonstrated ability to acquire and apply scientific knowledge and skills rapidly.

3. Exceptional oral and written communication skills with various talks at international conferences, published peer reviewed journal and conference papers.

Boston University, Boston, MA                                                                           4/2010 – Present

Post Doctoral Fellow, Biomedical Engineering Department

1.  Carrying out research to understand the deleterious effects of antibiotics on mammalian cells. The ultimate goal is to evaluate the long-term effects of antibiotics on mammalian cells and determine if prolonged usage would lead to mutagenesis using molecular biology techniques including RT²-PCR, genomic and mitochondrial DNA sequencing and microarray analysis on primary cells and in vivo mice studies.

2.  Additionally involved in the design and implementation of novel cell culture based experiments to validate the predictions made by mathematical modeling and computational studies of combinatorial drug therapy for cancer treatment.

Drexel University, Philadelphia, PA                                                                    9/2004 – 3/2010

Ph. D. Electrical and Computer Engineering, Mar 2010

PhD research specialized in understanding the physical and biological mechanisms of interaction of electrical non-thermal plasma with mammalian cells and tissues and developing clinical applications ranging from wound healing to cancer treatment.

Dissertation: “Mechanisms of Interaction of Non-Thermal Atmospheric Pressure Plasma with Mammalian Cells”

Veermata Jijabai Technological Institute (VJTI), Mumbai, India                             6/1999 – 6/2003

Bachelor of Engineering (BE), Electrical Engineering, Jun 2003

A. J. Drexel Plasma Institute   Graduate Research Fellow, PhD Candidate              4/2005 – Present

Drexel University, Philadelphia PA

1.  Investigated the induction of DNA damage by non-thermal plasma discharge in mammalian cells by looking at the phosphorylation of H2AX, a DNA damage marker. Additionally, using RNA interference and molecular biology techniques, studied the ATM/ATR mediated DNA repair pathways in response to DNA damage induced by non-thermal electrical plasma produced oxidative stress.

2.  Studied the induction of apoptosis in melanoma cancer cells by non thermal plasma electrical discharge by using Annexin-V/PI, Caspase cleavage and flow cytometry to develop non-thermal plasma as a novel clinical device for cancer treatment.

3.  Studied the possible short term and long-term toxic effects of the non-thermal plasma treatment on intact living tissue using in vivo porcine models utilizing immunohistochemistry and histological analysis of harvested pigskin and tissue.

4.  Studied the mechanisms of non-thermal plasma induced proliferation of endothelial cells to develop non-thermal plasma as a potential novel therapy for growth factor initiated promotion or inhibition of endothelial cell mediated angiogenesis, which is an important target for treating diseases in which there is either poor vascularization (chronic wounds) or abnormal vascularization (malignancies).

5.  Studied the physical and biological mechanisms of blood coagulation by non-thermal atmospheric pressure plasma.

Highly Commended Citation for the Drexel University Best Dissertation Award (2010); Highly Commended Citation for the Drexel University Excellence in Research Award (2010, 2009 and 2008); Graduate Student Outstanding Service Award (2009); Invited Talk, 36^th IEEE International Conference on Plasma Science (2009); IEEE Student Travel Award, 17^th IEEE International Pulsed Power Conference (2009); Honorable Mention for the PhD Level Student Paper Competition, ASME Summer Bioengineering Conference (2009); IEEE Student Travel Award, 36^th IEEE International Conference on Plasma Science (2009); Drexel University George Hill Jr. Endowed Fellowship (2009); Best Student Paper Award at the 36^th IEEE International Conference on Plasma Science (2008); Nominated for the Teaching Excellence Award (2008); Best Poster Award, "Technology with the Highest Translational Potential", The Translational Research in Biomedical Engineering Competition,  Drexel University (2008); NSF Student Travel Award, Gordon Research Conference on Plasma Processing Science (2008); Finalist for Best Student Paper Award, 35^th IEEE International Conference on Plasma Science (2007); Lee Smith Travelling Fellowship; 18^th International Symposium on Plasma Chemistry (2007); IEEE Student Travel Award, 35^th IEEE International Conference on Plasma Science (2007)

1. Effects of Non-Thermal Plasma on Mammalian Cells, S. Kalghatgi, C. Kelly, E. Cerchar, A. Fridman, G. Friedman, J. Azizkhan-Clifford. PLoS ONE, 21^st January 2011. 6(1): e16270. DOI: 10.1371/journal.pone.0016270. Online First

2.  Induction of Apoptosis in Melanoma Cells by Non-Thermal Atmospheric Pressure Plasma, R. Sensenig, S. Kalghatgi, E. Podolsky, E. Cerchar, G. Fridman, A. Shereshevsky, A. Fridman, G. Friedman, A. Brooks, J. Azizkhan-Clifford. Annals of Biomedical Engineering. 3^rd Nov 2010. 39(2): p 674 - 687.

3.  Endothelial Cell Proliferation is enhanced by Low Dose Non-Thermal Plasma Treatment through Fibroblast Growth Factor-2 Release, S. Kalghatgi, A. Fridman, G. Friedman, A. Morss Clyne. Annals of Biomedical Engineering, 2010. 38 (3): p 748 – 757

4.  Mechanism of Blood Coagulation by Non-Thermal Atmospheric Pressure Dielectric Barrier Discharge Plasma, S. Kalghatgi, G. Fridman, M. Cooper, G. Nagaraj, M. Peddinghaus, M. Balasubramanian, V. N. Vasilets, A. Gutsol, A. Fridman, G. Friedman, IEEE Transactions on Plasma Science, 2007. 35(5): p1559-1566.

1.  DNA Damage In Mammalian Cells By Non-Thermal Atmospheric Pressure Microsecond Pulsed Dielectric Barrier Discharge Plasma Is Not Mediated Via Lipid Peroxidation, S. Kalghatgi, J. Azizkhan-Clifford, A. Fridman, G. Friedman, 37th IEEE International Conference on Plasma Science (ICOPS), Jun 20 – 24, 2010, Norfolk, VA, USA.

2.  Non-Thermal Plasma Enhances Endothelial Cell Proliferation Through Fibroblast Growth Factor-2 Release, S. Kalghatgi, A. Fridman, A. Morss-Clyne, G. Friedman, 36th IEEE International Conference on Plasma Science (ICOPS), May 29th - Jun 4th 2009, San Diego, California, USA. (Invited)

3.  Non-Thermal Plasma Enhances Proliferation of Endothelial Cells, S. Kalghatgi, A. Fridman, Alisa Morss-Clyne, G. Friedman, 2^nd International Conference on Plasma Medicine (ICPM-2), Mar 16^th – Mar 20^th 2009, San Antonio, TX, USA. (Invited)

4.  Penetration of Direct Non-Thermal Plasma Treatment into Living Cells, S. Kalghatgi, C Kelly, G Fridman, A Fridman, J Azizkhan-Clifford, G Friedman, 35th IEEE International Conference on Plasma Science (ICOPS), June 15-19 2008, Karlsruhe, Germany (Best Student Paper Award)

5.  Mechanism of Blood Coagulation by Non-Thermal Atmospheric Pressure Dielectric Barrier Discharge Plasma, S. Kalghatgi, G. Fridman, M. Cooper, G. Nagaraj, M. Peddinghaus, M. Balasubramanian, V. Vasilets, A. Gutsol, A. Fridman, G. Friedman, 34^th IEEE International Conference on Plasma Science, June 17-22 2007, Albuquerque, New Mexico, USA

Laboratory Skills: RT²-PCR, Microarray Analysis, Genomic Sequencing, si/sh RNAi assays, Plasmid DNA extraction, Gel Electrophoresis, Immunoblotting (Western Blots), ELISA, Primary and Immortalized Cell Lines Culture, Cell Proliferation and Migration Assays, Apoptosis (caspase cleavage, TUNEL, Annexin V/PI), Immunohistochemistry, histology and FACS analysis, Fluorescence Microscopy, in-vivo animal studies (porcine and mice models), FTIR-ATR, SEM.

Software Tools: GraphPad Graphing & Statistics, Protein Quantification, Gel Imaging, MS Office, MATLAB, PSpice, ImagePro.

Research Writing: Journal and Conference manuscripts, IRB and IACUC protocols, grant proposals and patents

Treasurer                 Graduate Student Association (GSA)                                         6/2008 – 6/2009

Vice President      Engineering Graduate Association (EGA)                                        6/2006 – 6/2008

Secretary            Engineering Graduate Association (EGA)                                         6/2005 – 6/2006

Student member of American Association for Advancement of Science (AAAS)              1/2008 – Present

Student member of the Institute of Electrical and Electronic Engineers (IEEE)               9/2004 – Present

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