SAMEER KALGHATGI

           

Resume

 Sameer Kalghatgi, Ph. D.

 sameerkalghatgi@gmail.com

77 Crescent St, Apt 3, Waltham, MA 02453 Ÿ (267) 231-6595 Ÿ www.sameerkalghatgi.com

SENIOR SCIENTIST

Enthusiastic bioengineer with post doctoral experience having extensive hands-on skills in mammalian cell culture, stem cells, molecular cell biology, biochemistry, systems biology, immunology, signal transduction & animal models along with experience in conducting hypothesis driven multidisciplinary research is looking to lead R&D activities in drug discovery, novel pharmaceuticals and therapeutics at a major biotechnology firm.

 

SUMMARY

   Independent and innovative worker capable of working independently or in a team-centered multidisciplinary environment. Critical thinker with excellent analytical, organizational & project management skills along with a demonstrated ability to acquire and apply scientific knowledge and techniques rapidly.

   Independently setup a tissue culture and molecular biology lab to successfully lead a project on investigating the deleterious effects of common antibiotics on human cells using an integrated cellular-systems biology approach.

   Established transgenic and wild-type mice models to understand the physiological effects of acute and long-term antibiotic treatment and to develop a clinical solution to reduce or eliminate the detrimental effects of antibiotics.

   Excellent oral and written communication skills as evidenced by the various invited and contributed talks at international conferences and published first and co-authored papers in leading peer-reviewed journals.

 

SKILLS

   Adept in various biological techniques like mammalian cell culture, multi-parameter flow cytometry, FACS analysis, confocal and fluorescence microscopy; immunologic techniques including ELISA, gel electrophoresis, western blotting & immunofluorescence; multiplexed assays, RNAi gene knockdown, PCR, microarrays, DNA sequencing, DNA/RNA extraction, phenotypic characterization, signal transduction.

   Proficient in low, medium and high throughput cell-based assays for evaluating DNA damage, drug toxicity, cellular toxicity, oxidative stress, metabolism, mitochondrial function, proliferation & migration, apoptosis, angiogenesis.

   Highly skilled in establishing primary and immortalized cell lines including mouse & human ES/iPS cells.

   Extensive experience in designing and implementing wild-type and transgenic murine and porcine animal models.

   Trained in protein purification, colorimetric and fluorimetric assays, spectroscopy, HPLC and SEM.

 

WORK EXPERIENCE

Boston University, Department of Biomedical Engineering                                                       4/2010 – Present

Post Doctoral Research Associate with Dr. James Collins

Bactericidal Antibiotics induce Mitochondrial Dysfunction and Oxidative Stress in Mammalian Cells

   Investigated the underlying biological mechanisms of action of antibiotics on mammalian cells both in vitro and in vivo by studying DNA damage, oxidative stress, up/down regulation of key genes involved in antioxidant defenses, mitochondrial energy metabolism & mitochondrial dysfunction using advanced techniques in tissue culture, molecular cell biology, systems biology and biochemistry. Additionally, using qPCR and microarrays successfully studied the antibiotic induced changes in transcription at the whole genome level in mammalian cells.

   Closely involved in the design & implementation of novel cell culture based experiments to validate the predictions made by computational studies and mathematical modeling of combinatorial drug therapy for cancer treatment

Drexel University, ECE Department and A. J. Drexel Plasma Institute                                      9/2004 – 3/2010

Graduate Research Fellow with Dr. Gary Friedman and Teaching Assistant

Mechanisms of Interaction of Non-Thermal Plasma with Living Cells

   Independently investigated the biological and physical mechanisms of interaction of non-thermal plasma with mammalian cells. Additionally, using RNAi and advanced molecular biology techniques, studied the DNA repair pathways in response to damage induced by oxidative stress due to non-thermal plasma treatment.

   Took over the lead on a floundering project and successfully investigated the biological and cellular mechanisms induction of apoptosis in melanoma cells by electrical non-thermal plasma treatment by using various techniques such as Annexin-V/PI, Caspase cleavage, TUNEL and multi-parameter flow cytometry to develop non-thermal plasma as a novel clinical device for melanoma cancer treatment.

   Investigated the underlying mechanisms of plasma induced proliferation in endothelial cells to develop plasma as a potential therapy for promotion or inhibition of endothelial cell mediated angiogenesis, which is an important target for treating diseases where there is poor (chronic wounds) or abnormal (malignancies) vascularization.

   Established a porcine model to evaluate the intact and wounded skin responses to non-thermal plasma treatment.

 

EDUCATION

Drexel University, Philadelphia PA                                                                                          9/2004 – 3/2010

Ph. D. Electrical and Computer Engineering, Mar 2010

Dissertation: “Mechanisms of Interaction of Non-Thermal Plasma with Mammalian Cells”

 

Veermata Jijabai Technological Institute (VJTI), Mumbai, India                                                           6/1999 – 6/2003

Bachelor of Engineering (BE), Electrical Engineering, Jun 2003

 

AWARDS AND HONORS

Chair, Gordon Research Seminar on Plasma Processing Science (GRC 2012); Highly Commended Citation for the Drexel University Best Dissertation Award (2010); Highly Commended Citation for the Drexel University Excellence in Research Award (2010, 2009 and 2008); Graduate Student Outstanding Service Award (2009); Invited Talk, 37th IEEE ICOPS (2009); IEEE Student Travel Award, 17th IEEE International Pulsed Power Conference (2009); Drexel University George Hill Jr. Endowed Fellowship (2009); Best Student Paper Award at the 36th IEEE ICOPS (2008); NSF Student Travel Award, Gordon Research Conference on Plasma Processing Science (2010, 2008); Lee Smith Travelling Fellowship; 18th International Symposium on Plasma Chemistry (2007), IEEE Student Travel Award, 35th IEEE International Conference on Plasma Science (ICOPS 2007)

 

PUBLICATIONS

1. Bactericidal Antibiotics induce Mitochondrial Dysfunction and Oxidative Stress via a Common Mechanism in Mammalian Cells, S. Kalghatgi, J. Costello, C. Spina, A. Molina and J. J. Collins. (Under Review)

2. Effects of Non-Thermal Plasma on Mammalian Cells, S. Kalghatgi, et. al., PLoS ONE, 2011. 6(1): e16270.

3. DNA Damage in Mammalian Cells by Atmospheric Pressure Microsecond Pulsed DBD Plasma is not Mediated by Ozone. S. Kalghatgi, et. al., Plasma Processes Polym., 2012, doi: 10.1002/ppap.201100156

4. DNA Damage in Mammalian Cells by Atmospheric Pressure Microsecond Pulsed DBD Plasma is not Mediated via Lipid Peroxidation, S. Kalghatgi and J. Azizkhan-Clifford. Plasma Medicine, (In Press).

5. Endothelial Cell Proliferation is enhanced by Low Dose Non-Thermal Plasma Treatment through FGF-2 Release, S. Kalghatgi, et. al., Annals Biomed. Engg, 2010. 38 (3): p 748 – 757.

6. Mechanism of Blood Coagulation by Non-Thermal Atmospheric Pressure DBD Plasma, S. Kalghatgi, et. al., IEEE Transactions on Plasma Science, 2007. 35(5): p1559-1566.

 

SELECTED CONFERENCE TALKS

1. Bactericidal Antibiotics Induce Oxidative Stress in Mammalian Cells via Mitochondrial Dysfunction, S. Kalghatgi, et. al., BMES Annual Meeting, Oct 12 – 15th 2011, Hartford, CT, USA.

2. Non-Thermal Plasma Enhances Endothelial Cell Proliferation Through Fibroblast Growth Factor-2 Release, S. Kalghatgi, et. al., 36th IEEE ICOPS, May 29th - Jun 4th 2009, San Diego, California, USA. (Invited Talk).

3. Penetration of Direct Non-Thermal Plasma Treatment into Living Cells, S. Kalghatgi, et. al., 35th IEEE International Conference on Plasma Science (ICOPS), June 15-19 2008, Karlsruhe, Germany (Best Student Paper Award).

4. Mechanism of Blood Coagulation by Non-Thermal Atmospheric Pressure DBD Plasma, S. Kalghatgi, et. al., 34th IEEE International Conference on Plasma Science, June 17-22 2007, Albuquerque, New Mexico, USA. (Travel Award)

 

LEADERSHIP & ORGANIZATIONAL ACTIVITIES

1.       Chair                             Gordon Research Seminar – Plasma Processing Science                 Jul 20th – 21st 2012

2.       Treasurer                      Graduate Student Association (GSA)                                                  6/2008 – 6/2009

3.       Vice President               Engineering Graduate Association (EGA)                                          6/2006 – 6/2008

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Publications

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Areas of Research   

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Current Happenings

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